e2 conjugase (R&D Systems)
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e2 conjugase
E2 Conjugase, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 59 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/e2+conjugase/Recombinant+Human%2FMouse%2FRat+UbcH5c%2FUBE2D3+Protein%2C+CF/pmc11602139-122-53-57
Average 95 stars, based on 59 article reviews
E2 Conjugase, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 59 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/e2+conjugase/Recombinant+Human%2FMouse%2FRat+UbcH5c%2FUBE2D3+Protein%2C+CF/pmc11602139-122-53-57
Average 95 stars, based on 59 article reviews
e2 conjugase - by Bioz Stars,
2026-09
95/100 stars
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Ubiquitin Proteomics:Article Title: PCNA-binding activity separates RNF168 functions in DNA replication and DNA double-stranded break signaling Article Snippet: Analysis of the isotherm yielded K d = 76.3 nM and stoichiometry = 0.93 (Microcal Origin software). .. Ubiquitylation assays were performed in 25 μl reactions in which the components were added in the following order: dd H2O, 1× Energy regeneration solution (#B-10 R&D systems), 75 mM ubiquitin (#U-100H R&D systems), 1.6 mM FLAG-PCNA substrate (expressed and purified in bacteria), 0.1 mM E1 Ubiquitin Activating Enzyme (#E-304 R&D systems), 0.2 mM Article Title: A Degenerate PCNA-Interacting Peptide (DPIP) box targets RNF168 to replicating DNA to limit 53BP1 signaling Article Snippet: Analysis of the isotherm yielded Kd = 76.3 nM and stoichiometry = 0.93 (Microcal Origin software). .. Ubiquitylation assays were performed in 25 mL reactions in which the components were added in the following order: dd H2O, 1x Energy regeneration solution (#B-10 R&D systems), 75 mM ubiquitin (#U-100H R&D systems) 1.6 mM FLAG-PCNA substrate (expressed and purified in bacteria), 0.1 mM E1 Ubiquitin Activating Enzyme (#E-304 R&D systems), 0.2 mM Article Title: PCNA-binding activity separates RNF168 functions in DNA replication and DNA double-stranded break signaling. Article Snippet: .. D ow nloaded from https://academ ic.oup.com /nar/advance-article/doi/10.1093/nar/gkae918/7833678 by guest on 02 N ovem ber 2024 D ow nloaded from https://academ ic.oup.com /nar/advance-article/doi/10.1093/nar/gkae918/7833678 by guest on 02 N ovem ber 2024 In vitro ubiquitylation assay Ubiquitylation assays were performed in 25 μl reactions in which the components were added in the following order: dd H2O, 1 × Energy regeneration solution (#B-10 R&D systems), 75 mM ubiquitin (#U-100H R&D systems), 1.6 mM FLAGPCNA substrate (expressed and purified in bacteria), 0.1 mM E1 Ubiquitin Activating Enzyme (#E-304 R&D systems), 0.2 mM Purification:Article Title: PCNA-binding activity separates RNF168 functions in DNA replication and DNA double-stranded break signaling Article Snippet: Analysis of the isotherm yielded K d = 76.3 nM and stoichiometry = 0.93 (Microcal Origin software). .. Ubiquitylation assays were performed in 25 μl reactions in which the components were added in the following order: dd H2O, 1× Energy regeneration solution (#B-10 R&D systems), 75 mM ubiquitin (#U-100H R&D systems), 1.6 mM FLAG-PCNA substrate (expressed and purified in bacteria), 0.1 mM E1 Ubiquitin Activating Enzyme (#E-304 R&D systems), 0.2 mM Article Title: A Degenerate PCNA-Interacting Peptide (DPIP) box targets RNF168 to replicating DNA to limit 53BP1 signaling Article Snippet: Analysis of the isotherm yielded Kd = 76.3 nM and stoichiometry = 0.93 (Microcal Origin software). .. Ubiquitylation assays were performed in 25 mL reactions in which the components were added in the following order: dd H2O, 1x Energy regeneration solution (#B-10 R&D systems), 75 mM ubiquitin (#U-100H R&D systems) 1.6 mM FLAG-PCNA substrate (expressed and purified in bacteria), 0.1 mM E1 Ubiquitin Activating Enzyme (#E-304 R&D systems), 0.2 mM Article Title: PCNA-binding activity separates RNF168 functions in DNA replication and DNA double-stranded break signaling. Article Snippet: .. D ow nloaded from https://academ ic.oup.com /nar/advance-article/doi/10.1093/nar/gkae918/7833678 by guest on 02 N ovem ber 2024 D ow nloaded from https://academ ic.oup.com /nar/advance-article/doi/10.1093/nar/gkae918/7833678 by guest on 02 N ovem ber 2024 In vitro ubiquitylation assay Ubiquitylation assays were performed in 25 μl reactions in which the components were added in the following order: dd H2O, 1 × Energy regeneration solution (#B-10 R&D systems), 75 mM ubiquitin (#U-100H R&D systems), 1.6 mM FLAGPCNA substrate (expressed and purified in bacteria), 0.1 mM E1 Ubiquitin Activating Enzyme (#E-304 R&D systems), 0.2 mM Bacteria:Article Title: PCNA-binding activity separates RNF168 functions in DNA replication and DNA double-stranded break signaling Article Snippet: Analysis of the isotherm yielded K d = 76.3 nM and stoichiometry = 0.93 (Microcal Origin software). .. Ubiquitylation assays were performed in 25 μl reactions in which the components were added in the following order: dd H2O, 1× Energy regeneration solution (#B-10 R&D systems), 75 mM ubiquitin (#U-100H R&D systems), 1.6 mM FLAG-PCNA substrate (expressed and purified in bacteria), 0.1 mM E1 Ubiquitin Activating Enzyme (#E-304 R&D systems), 0.2 mM Article Title: A Degenerate PCNA-Interacting Peptide (DPIP) box targets RNF168 to replicating DNA to limit 53BP1 signaling Article Snippet: Analysis of the isotherm yielded Kd = 76.3 nM and stoichiometry = 0.93 (Microcal Origin software). .. Ubiquitylation assays were performed in 25 mL reactions in which the components were added in the following order: dd H2O, 1x Energy regeneration solution (#B-10 R&D systems), 75 mM ubiquitin (#U-100H R&D systems) 1.6 mM FLAG-PCNA substrate (expressed and purified in bacteria), 0.1 mM E1 Ubiquitin Activating Enzyme (#E-304 R&D systems), 0.2 mM Article Title: PCNA-binding activity separates RNF168 functions in DNA replication and DNA double-stranded break signaling. Article Snippet: .. D ow nloaded from https://academ ic.oup.com /nar/advance-article/doi/10.1093/nar/gkae918/7833678 by guest on 02 N ovem ber 2024 D ow nloaded from https://academ ic.oup.com /nar/advance-article/doi/10.1093/nar/gkae918/7833678 by guest on 02 N ovem ber 2024 In vitro ubiquitylation assay Ubiquitylation assays were performed in 25 μl reactions in which the components were added in the following order: dd H2O, 1 × Energy regeneration solution (#B-10 R&D systems), 75 mM ubiquitin (#U-100H R&D systems), 1.6 mM FLAGPCNA substrate (expressed and purified in bacteria), 0.1 mM E1 Ubiquitin Activating Enzyme (#E-304 R&D systems), 0.2 mM Recombinant:Article Title: PCNA-binding activity separates RNF168 functions in DNA replication and DNA double-stranded break signaling Article Snippet: Analysis of the isotherm yielded K d = 76.3 nM and stoichiometry = 0.93 (Microcal Origin software). .. Ubiquitylation assays were performed in 25 μl reactions in which the components were added in the following order: dd H2O, 1× Energy regeneration solution (#B-10 R&D systems), 75 mM ubiquitin (#U-100H R&D systems), 1.6 mM FLAG-PCNA substrate (expressed and purified in bacteria), 0.1 mM E1 Ubiquitin Activating Enzyme (#E-304 R&D systems), 0.2 mM Article Title: A Degenerate PCNA-Interacting Peptide (DPIP) box targets RNF168 to replicating DNA to limit 53BP1 signaling Article Snippet: Analysis of the isotherm yielded Kd = 76.3 nM and stoichiometry = 0.93 (Microcal Origin software). .. Ubiquitylation assays were performed in 25 mL reactions in which the components were added in the following order: dd H2O, 1x Energy regeneration solution (#B-10 R&D systems), 75 mM ubiquitin (#U-100H R&D systems) 1.6 mM FLAG-PCNA substrate (expressed and purified in bacteria), 0.1 mM E1 Ubiquitin Activating Enzyme (#E-304 R&D systems), 0.2 mM Article Title: PCNA-binding activity separates RNF168 functions in DNA replication and DNA double-stranded break signaling. Article Snippet: .. D ow nloaded from https://academ ic.oup.com /nar/advance-article/doi/10.1093/nar/gkae918/7833678 by guest on 02 N ovem ber 2024 D ow nloaded from https://academ ic.oup.com /nar/advance-article/doi/10.1093/nar/gkae918/7833678 by guest on 02 N ovem ber 2024 In vitro ubiquitylation assay Ubiquitylation assays were performed in 25 μl reactions in which the components were added in the following order: dd H2O, 1 × Energy regeneration solution (#B-10 R&D systems), 75 mM ubiquitin (#U-100H R&D systems), 1.6 mM FLAGPCNA substrate (expressed and purified in bacteria), 0.1 mM E1 Ubiquitin Activating Enzyme (#E-304 R&D systems), 0.2 mM In Vitro:Article Title: PCNA-binding activity separates RNF168 functions in DNA replication and DNA double-stranded break signaling. Article Snippet: .. D ow nloaded from https://academ ic.oup.com /nar/advance-article/doi/10.1093/nar/gkae918/7833678 by guest on 02 N ovem ber 2024 D ow nloaded from https://academ ic.oup.com /nar/advance-article/doi/10.1093/nar/gkae918/7833678 by guest on 02 N ovem ber 2024 In vitro ubiquitylation assay Ubiquitylation assays were performed in 25 μl reactions in which the components were added in the following order: dd H2O, 1 × Energy regeneration solution (#B-10 R&D systems), 75 mM ubiquitin (#U-100H R&D systems), 1.6 mM FLAGPCNA substrate (expressed and purified in bacteria), 0.1 mM E1 Ubiquitin Activating Enzyme (#E-304 R&D systems), 0.2 mM Ubiquitin Assay:Article Title: PCNA-binding activity separates RNF168 functions in DNA replication and DNA double-stranded break signaling. Article Snippet: .. D ow nloaded from https://academ ic.oup.com /nar/advance-article/doi/10.1093/nar/gkae918/7833678 by guest on 02 N ovem ber 2024 D ow nloaded from https://academ ic.oup.com /nar/advance-article/doi/10.1093/nar/gkae918/7833678 by guest on 02 N ovem ber 2024 In vitro ubiquitylation assay Ubiquitylation assays were performed in 25 μl reactions in which the components were added in the following order: dd H2O, 1 × Energy regeneration solution (#B-10 R&D systems), 75 mM ubiquitin (#U-100H R&D systems), 1.6 mM FLAGPCNA substrate (expressed and purified in bacteria), 0.1 mM E1 Ubiquitin Activating Enzyme (#E-304 R&D systems), 0.2 mM |